INFLUENCE OF CULTURE MEDIA ON ANTIFUNGAL ACTIVITY PRODUCED BY STREPTOMYCES SP. PAL114 ISOLATED FROM GHARDAÏA DATE PALM GROVE SOILS

Abstract

An actinobacterial strain, named PAL114, isolated from date palm grove soil of Ghardaïa (Algeria)  showed an important antifungal activity against various pathogenic and/or mycotoxigenic fungi on  different culture media. The highest level of antifungal activity was observed after the 12th day of  fermentation. The Bennett medium was found to be the best medium for optimum growth and antifungal  activity. The produced metabolites were detected by reverse-phase HPLC using a C18 column and the  fractions corresponding to peaks were collected, concentrated then tested against Aspergillus carbonarius M333 to detect the active fractions. The results showed the presence of novel bioactive metabolites on the  12th day, which exhibited significant antifungal activity