EFFET DU STRESS SALIN SUR LA GERMINATION DES CARYOPSES D’ORGE MARITIME (HORDEUM MARINUM HUDS.)

Abstract

The objective of this work was to evaluate the effect of salt stress on the germinative behavior of sea  barley seedes (Hordeum marinum Huds.). Before germination, the seeds were pretreated with cold (stratification) - at 4 ° C - for two weeks, then  disinfected by sodium hypochlorite for one minute and soaked in distilled water during one hour. Aft er  disinfection and soaking, ten seeds were placed in Petri dishes covered with filter paper. 12 Petri dishes  were placed at room temperature under a photoperiod of 16 hours light and 8 hours dark and twelve other Petri dishes were placed in the dark at room temperature. The moistening of filter paper was  conducted daily with 0, 150 and 300 mM NaCl solutions. These steps were repeated with non-pretreated  seeds. Germination was recorded every 24 hours for each set during a period of 15 days. Two parameters  were considered in this test, the daily germination rate and the cumulative germination rate. The results showed that salinity influenced the germination capacity of sea barley seeds. NaCl  significantly reduced germination rate, germination kinetics, and cumulative germination rate (subjected  to 16 hours light and 8 hours dark or to dark, stratified or non -stratified).